應(yīng)用
Y(II)或ΔF/Fm’ 或 (Fm’ – Fs )/Fm’) 是經(jīng)受時(shí)間考驗(yàn)的光適應(yīng)測(cè)量參數(shù)，比Fv/Fm對(duì)更多類型的植物脅迫更加敏感。已有的大量證據(jù)表明Fv/Fm對(duì)許多種植物脅迫和健康植物的光系統(tǒng)II的測(cè)量十分出色，而Y(II)或光量子產(chǎn)額則可測(cè)量實(shí)際光照下光適應(yīng)環(huán)境和生理狀況的光系統(tǒng)II的效率。
原理
采用調(diào)制飽和脈沖原理，測(cè)量植物的葉綠素?zé)晒猓瑴y(cè)量參數(shù)包括植物的光量子產(chǎn)額Y(II)及相對(duì)電子傳遞速率ETR，最大光化學(xué)效率Fv/Fm，同時(shí)還可測(cè)量PAR、葉溫、相對(duì)濕度和葉片吸光率等環(huán)境參數(shù)。
特點(diǎn)
葉片吸光率測(cè)量：提供葉片吸收測(cè)量及隨環(huán)境變化導(dǎo)致的葉片吸收變化。根據(jù)Eichelman (2004) 葉片吸收在健康植物的變化范圍在0.7~0.9 之間。因此，為獲得準(zhǔn)確的ETR或“J”，Y(II)測(cè)量?jī)x提供了一個(gè)可靠的測(cè)量方法，
Fv/Fm測(cè)量單元：用于暗適應(yīng)測(cè)量。
先進(jìn)的PAR葉夾：采用底部葉夾打開(kāi)裝置，防止測(cè)量時(shí)誤操作打開(kāi)葉夾。對(duì)傳感器進(jìn)行余弦校正，確保葉片相對(duì)測(cè)量光的角度不變。
Fm’校正：對(duì)于具有高光照強(qiáng)度歷史的植物，*關(guān)閉光反應(yīng)中心是一個(gè)問(wèn)題，Y(II)測(cè)量?jī)x使用Loriaux &Genty 2013的方法進(jìn)行Fm’ 校正，確保可以測(cè)得準(zhǔn)確的Fm’ 。
自動(dòng)調(diào)制光設(shè)定：快速準(zhǔn)確自動(dòng)的調(diào)整合適的調(diào)制光強(qiáng)，避免人工操作的誤差。
先進(jìn)算法避免飽和脈沖NPQ：采用25ms內(nèi)8點(diǎn)的平均值確定Fm、Fm’、Fo、Fs，消除飽和脈沖NPQ的影響和電子噪音。
更精確的葉溫測(cè)量：采用非接觸式紅外測(cè)量，測(cè)量精度可達(dá)±0.5℃。
直接測(cè)量相對(duì)濕度：含有測(cè)量氣體交換使用的固態(tài)傳感器，可測(cè)量相對(duì)濕度。
降低葉片遮擋的設(shè)計(jì)：傾斜的角度減少對(duì)葉片的遮擋，可以測(cè)量擬南芥等小葉。
系統(tǒng)組成
標(biāo)配： Y(II)光量子產(chǎn)額測(cè)量?jī)x，F(xiàn)v/Fm測(cè)量?jī)x及10個(gè)暗適應(yīng)葉夾，2個(gè)電池，2個(gè)充電器，一個(gè)便攜箱，文件U盤。
技術(shù)指標(biāo)
測(cè)量參數(shù)：
Y(II)或ΔF/Fm‘、ETR、PAR、Tleaf、相對(duì)濕度、Fms或Fm’、Fs、α(葉片吸收率)、FV/FM、FV/FO，F(xiàn)O, FM, FV。
監(jiān)測(cè)模式：允許長(zhǎng)時(shí)間監(jiān)測(cè)
技術(shù)參數(shù)：
Y(II): 光適應(yīng)測(cè)量, 穩(wěn)態(tài)光合作用下的環(huán)境光
光源
飽和脈沖： LED白光源，使用PAR葉夾時(shí)可達(dá)7000μmols
調(diào)制光：紅光，LED 660nm，具有690nm窄通過(guò)濾器。
光化光源：環(huán)境光
檢測(cè)方法：脈沖調(diào)制法
PAR：測(cè)量400-700nm，余弦校正 ±2umols
Fv/Fm：暗適應(yīng)測(cè)量
光源：LED紅光飽和光閃，可達(dá)6000umols；
調(diào)制光：660nmLED 紅光，690nm濾波器
調(diào)制光可以根據(jù)實(shí)際測(cè)量自動(dòng)調(diào)節(jié)到合適的強(qiáng)度，減少手動(dòng)調(diào)節(jié)誤差，
相對(duì)濕度：0%~100%，±2%。
檢測(cè)器&過(guò)濾器：具有700~750nm帶通過(guò)濾的PIN光電二極管
可選配三腳架。
顯示：132 X 30 pixel 液晶顯示屏
取樣速率：1~10000點(diǎn)/秒自動(dòng)切換。
測(cè)量時(shí)間：最短3s或也可設(shè)置長(zhǎng)期監(jiān)測(cè)模式
存儲(chǔ)空間：2GB
輸出：USB下載數(shù)據(jù)，用Excel查看，無(wú)需安裝其他專用軟件
供電：USB鋰離子電池(普通充電寶)，可用8小時(shí)
尺寸：便攜箱尺寸為14”x 11”x 6”，儀器為9’’長(zhǎng)
質(zhì)量：Y(II) 測(cè)量?jī)x0.45 kg
Fv/Fm測(cè)量?jī)x0.36 kg.
加便攜箱和附件總重1.95 kg.
工作溫度：0℃ ~ 50℃
產(chǎn)地
美國(guó)
文獻(xiàn)
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